00:09:10 Paul Hutchinson: Hi everyone 00:09:30 Widiasih TK, ICA Committee: hi Paul 00:09:36 Lyana Setiawan: hi all 00:10:30 Widiasih TK, ICA Committee: would you make us as co host ? 00:11:47 Paul Hutchinson: Yes 00:12:10 Paul Hutchinson: I am just going to have breakfast. Back in 10 mins 01:33:29 putri chadijah: To Prof Wendy, so Immune-flow FISH is using flowcytometry sample after Monoclonal antibody added and we performed FISH?in the same machine? 01:52:09 Paul Hutchinson: Please type any questions you have for Prof Wendy and Prof Kathy here 01:52:13 Alejandro Arevalo: Very interesting topic, Dr Erber. Question: How do you establish the cut off values for each probe in immuno FLOW FISH? 2. In patient who previously received daratumumab, can you label plasma cells in MM by CD38 alone? 01:54:49 Hafiza Alauddin: Thank you very much Prof Erber and Dr Fuller for the very enlightening talks. How did your immunoFISH compared to the regular FISH results so far? 01:59:09 Sindhu Cherian: Thanks for the great talks on an exciting topic! Are you currently doing this on a clinical basis? Also, how long does the process take per patient sample from start to finish? 02:00:19 Yulia Nadar Indrasari: To Prof. Erber, question: in MDS del(5q), is it possible to be detected with immunoflow FISH? 02:00:27 Liu yixian: In the setting of CML, molecular test of BCR-ABL is already very sensitive. Do we still need the FISH-flow for diagnostic/prognostic purpose? 02:00:33 Alejandro Arevalo: Thank you very much, professor Erber 02:00:38 Mardziah binti Mohamad: Hi Profs... I would like to know.. if we were to adopt this method of analysis into our centre.. can u advice on the basic equipment/probes needed.. 02:05:10 Hafiza Alauddin: yes…I can hear everyone clearly 02:23:30 TPO5 Halimatun Radziah: the first aspirate is always everyone's choice, as tubes will go to different test, which test is the most priority for the first aspirate. i have difficulties in prioritising specimen for tests (IPT, chromosome analysis, molecular) because marrow aspirate can easily get clotted depending on patient's condition and difficulty encountered during bma procedure. 02:26:52 Lyana Setiawan: how about body fluids such as cerebrospinal fluid? 02:28:27 shafini: what is the minimum WBC for body fluid (eg CSF) to proceed with flow cytometry analysis 02:28:33 Hafiza Alauddin: Thank you for your talk. Is advised to use the first draw prioritised for IPT over morphology now? 02:32:06 Veronika Juanita: Is there any preservatives that able to prolong the stability of EDTA sample?or is there any other effort that we can do to prolong the stability? Since we often receive samples from very remote area. Thank you 02:39:37 Liu yixian: In the setting of PNH flow, will the sample that arrived after 24 hours get degraded signals in relation to Cd16 and Flaer? 02:40:28 TPO5 Halimatun Radziah: ms Veronika, i agree. sometimes bma collection can drag time and causing sample to arrive late at the laboratory. this usually occurs to centre that outsource the specimen to outsourced laboratory. if there's any preservative that can be added to the stability, that would be great as some cellls like plasma cells are fragile 02:40:32 Pek Kuen Liew: Do u run all csf samples for immunophenotyping regardless of count? 02:46:12 Pek Kuen Liew: Thank you for the excellent explanation and very practical. 02:48:50 Veronika Juanita: yes Ms Halimatun and we have to be able to distribute such preservatives to the area 02:54:08 dr Isti Noviani SpPK: thank you for the interesting topiks.. 02:56:30 Audrey Paulus: yes we can hear you 03:31:31 Lyana Setiawan: To Maggie, will you recommend using third party commercial QC material for clinical tests such as 03:32:16 Lyana Setiawan: clinical tests such as hematopoietic stem cell enumeration or CD4 or TBNK testing? 03:36:40 Pek Kuen Liew: R the rainbow peak beads run daily in addition to instrument qc beads in ur centre? 03:38:44 Verdiansah: is it enough to do QC that recommended or made for our instrument? 03:39:46 Amanda: Dear Professor / Doctor / Sir / Madam, Kindly click on the following link and help to fill in this quick 3 min survey : https://qurl.one2ten.com/sysmex-asia-pacific-26-may-chat Sysmex appreciates your support. Thank you... 03:52:46 Amanda: Dear Professor / Doctor / Sir / Madam, Kindly click on the following link and help to fill in this quick 3 min survey : https://qurl.one2ten.com/sysmex-asia-pacific-26-may-chat Sysmex appreciates your support. Thank you... 03:53:57 leekien: Hi udrey, may i know what is the regulatory status of this flow cytometer? 03:54:02 Audrey Paulus: thank you everyone ! 03:54:11 2_dr Budiono Raharjo, M.Th,Sp.PK (K): thankyou 03:54:15 sammycheang: thank you and have a nice day